Our laboratory takes a comparative medicine approach to studying human disorders such as polycystic kidney disease and inflammatory bowel disease by using a variety of animal models from rodents to zebrafish. We are interested in characterizing disease-causing genes and their protein products in order to elucidate the molecular pathways in which these genes/proteins participate. This knowledge will allow a better understanding of both normal and abnormal development and may ultimately lead to targeted therapeutics. Additionally, we are interested in the generation and characterization of new animal models. Towards this, our lab has characterized rat embryonic stem cells (ESCs), and isolated new rat ESC lines. We have helped validate a novel rat and zebrafish cell ablation model system and we use evolving technologies such as CRISPR/Cas9 to create new genetically engineered animal models. Genome editing has allowed us to create rodent models that carry rare human genetic alterations in order to validate these putative disease-causing mutations and generate animal models that can facilitate personalized medicine interventions. We are also interested in studying the role of the microbiome on animal model phenotypes, including using zebrafish models of stress and anxiety, investigating microbiota sex differences in an Alzheimer’s Disease rat model and evaluating the impact of probiotics in a variety of animal model systems.
Bryda, E.C., Men, H., Davis, D.J., Bock, A.S., Shaw, M.L., Chesney, K.L., Hankins, M.A. A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression. Sci Report 9:13330 (2019).
Shimoyama, M., Smith, J.R., Bryda, E., Kuramoto, T., Saba, L., Dwinell, M. Rat Genome and Model Resources. ILAR J. 58:42-58 (2017).
Walters, E.M., Wells, K.D., Bryda, E.C., Schommer, S., Prather, R.S. Swine models, genomic tools and services to enhance our understanding of human health and diseases. Lab Anim (NY). 46:167-172 (2017).
Li, S., Lan, H., Men, H., Wu, Y., Li, N., Capecchi, M.R., Bryda, E.C., Wu, S. Derivation of Transgene-Free Rat Induced Pluripotent Stem Cells Approximating the Quality of Embryonic Stem Cells. Stem Cells Transl Med. 6:340-351 (2017).
Lan, H., Li, S., Guo, Z., Men, H., Wu,Y., Li, N., Bryda, E.C., Capecchi, M.R., Wu, S. Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells. FEBS Lett. 590:3416-3424 (2016).
Davis, D.J., Doerr, H.M., Grzelak, A.K., Busi, S.B., Jasarevic, E., Ericsson, A.C., Bryda, E.C. Lactobacillus plantarum attenuates stress-induced responses in adult zebrafish via the gut-brain axis. Scientific Reports 6:33726 (2016).
Hanson, M.M., Liu, F., Dai, S., Kearns, A., Qin, X., Bryda, E.C. Rapid conditional ablation model for hemolytic anemia in the rat. Physiological Genomics 48:626-632 (2016).
Ericsson, A.C., Akter, S., Hanson, M.M., Busi, S.B., Parker, T.W., Schehr, R.J., Hankins, M.A., Ahner, C.E., Davis, J.W., Franklin, C.L., Amos-Landgraf, J.M., Bryda, E.C. Differential Susceptibility to Colorectal Cancer due to Naturally Occurring Gut Microbiota. Oncotarget 6:33689-33704 (2016).
Taskiran, E.Z., Korkmaz, E., Gucer, S., Kosukcu, C., Kaymaz, F., Koyunlar, C., Bryda, E.C., Chaki, M., Lu, D., Vadnagara, K., Candan, C., Topaloglu, R., Schaefer, F., Attanasio, M., Bergmann, C., Ozaltin, F. Mutations in ANKS6 Cause a Nephronophthisis-Like Phenotype with ESRD. J Am Soc Nephrol. 25:1653-1661 (2014).
Bock, A.S., Leigh, N.D., Bryda, E.C. Effect of Gsk3 inhibitor CHIR99021 on aneuploidy levels in rat embryonic stem cells. In Vitro Cell Dev Biol Anim. 50:572–579 (2014).
Men, H. and Bryda, E.C. Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line. PLoS One 8:e56518 (2013).
Men, H., Bauer, B.A., Bryda, E.C. Germline Transmission of a Novel Rat Embryonic Stem Cell Line Derived From Transgenic Rats. Stem Cells and Development 21:2606-2612 (2012).
Bryda, E.C., Johnson, N.T., Ohlemiller, K.K., Besch-Williford, C.L., Moore, E., Bram, R.J. Conditional Deletion of Calcium-modulating Cyclophilin Ligand Causes Deafness in Mice. Mamm Genome 23:270-276 (2011).
Hillhouse A., Taylor J., Bryda E.C., Myles M. and Franklin C.F. Identification of quantitative trait loci on mouse Chromosome 3 and 17. Mamm Genome 22:544–555 (2011).
McElroy J.A., Bryda E.C., McKay, S.D., Schnabel R.D., Taylor J.F. Genetic Variation at a Metallothionein 2A Promoter Single Nucleotide Polymorphism in White and Black females. Journal of Toxicology and Environmental Health, Part A 73:1283-1287 (2010)
Bouvrette, D.J., Sittaramane, V., Heidel, J.R., Chandrasekhar, A., Bryda, E.C. Knockdown of Bicaudal C in Zebrafish (Danio rerio) Causes Cystic Kidneys: A Non-Mammalian Model of Polycystic Kidney Disease. Comparative Medicine 60:96-106 (2010).
Bryda, E.C. and Bauer, B.A. Chapter 20: A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites. In “Methods in Molecular Biology, Rat Genomics: Gene identification, Functional Genomics and Model Applications.” I. Anegon (ed). Humana Press, Totowa, NJ. Methods Mol Biol. 597:287-299 (2010).
Walters, E.M., Bauer, B.A., Franklin, C.L., Evans, T.J., Bryda, E.C., Riley, L.K., Critser, J.K. Mutational Insertion of a ROSA26-EGFP Transgene Leads to Spermiogenesis Defects and Male Infertility in Mice. Comparative Medicine 59: 1-8 (2009).
Black, J., Dykes, A., Thatcher, S., Brown, D., Bryda, E.C., Wright, G.L. FRET Analysis of Actin/Myosin Interaction in Contracting Aortic Smooth Muscle. The Canadian Journal of Physiology and Pharmacology 87: 327-336 (2009).
Stagner, E.E., Bouvrette, D.J., Cheng, J., Bryda, E.C. The Polycystic Kidney Disease-related Proteins Bicc1 and SamCystin Interact. Biochemical and Biophysical Research Communications 383: 16-21 (2009).
Bryda, E.C. and Riley, L.K. Multiplex Microsatellite Markers Panels for Genetic Monitoring of Common Rat Strains. Journal of the American Association for Laboratory Animal Science 47: 37-41 (2008).
Bouvrette, D.J., Price, S.J., Bryda, E.C. The K Homology Domains of the Mouse Polycystic Kidney Disease-related Protein, Bicaudal-C (Bicc1) Mediate RNA Binding In Vitro. Nephron Experimental Nephrology 108:27-34 (2008).
Bauer, B.A., Boedges, S., Cook, C.R., Bryda, E.C., Franklin, C. L. Breeding Colony Refinement Through Phenotypic and Genotypic Characterization of the SPRD-Pkdr1/Rrrc Rat Model of Polycystic Kidney Disease. Comparative Medicine 57:193-199 (2007).
Bryda, E.C., Pearson, M., Agca, Y., Bauer, B.A. Method for Detection and Identification of Multiple Chromosomal Integration Sites in Transgenic Animals Created with Lentivirus. Biotechniques 41:715-719 (2006).
Cogswell, C., Price, S. J., Hou, X., Guay-Woodford, L.M., Flaherty, L., Bryda, E. C. Positional cloning of the jcpk/bpk locus of the mouse. Mammalian Genome 14:242-249 (2003).
Price, S.J., Chittenden, L., Flaherty, L., O’Dell, B., Guay-Woodford, L.M., Stubbs, L., Bryda, E.C. Characterization of the Region Containing the jcpk PKD Gene on Mouse Chromosome 10. Cytogenetics and Genome Research 98:61-66 (2002).
Bryda, E.C., Kim, H.J., Legare, M.E., Frankel, W., Noben-Trauth, K. High resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and waltzer (v). Genomics 73: 338-342 (2001).
Di Palma, F., Holme, R.H., Bryda, E.C., Belyantseva, I.A., Pellegrino, R., Kachar, B., Steel, K.P., Noben-Trauth, K. Mutations in Cdh23, encoding a new type of cadherin, cause stereocilia disorganization in waltzer, the mouse model for Usher syndrome type 1D. Nature Genetics 27: 103-107 (2001).
Bolz, H., von Brederlow, B., Ramirez, A., Bryda, E.C., Kutsche, K., Nothwang, H-G, Seeliger, M., Cabrera, M. d.C-S., Vila, M.C., Molina, O.P., Gal, A., Kubisch, C. Mutation of CDH23, encoding a new member of the cadherin gene family, causes Usher syndrome type 1D. Nature Genetics 27:108-112 (2001).