John Driver

Associate Professor of Animal Sciences

Animal Sciences,

driverjp@missouri.edu

Research Emphasis

My research focuses on livestock immunology with an emphasis on understanding the contribution of innate T cells to immunity against influenza viruses and other pathogens important for swine and human health. In this pursuit, I work with MU’s National Swine Research and Resource Center to produce pigs genetically lacking different subsets of T and B cells to understand their contribution to mucosal immune responses. I am also heavily invested in single cell transcriptomics technology which is extremely powerful for identifying types and subtypes of cells as well as regulatory networks that affect cell function. This platform offers tremendous opportunities to understand immune responses in livestock species for which relatively few immune reagents are available. In addition to his work in livestock immunology, I has > 15 years of T1D research experience with a focus on identifying and characterizing genes involved in autoreactive T cell development. I recently identified mouse mammary tumor provirus 3 as an underlying T1D resistance gene in the T1D susceptible NOD mouse, the primary animal model for studying human T1D.

Research Interests

• Immunology
• Virology
• Livestock genetics

Education

PhD 2004 Animal Nutrition – University of Georgia, Athens, GA
MS 2001 Animal Nutrition – University of Pretoria, South Africa
BS 1998 Animal Science – University of Pretoria, South Africa

Selected Publications

Artiaga BL, Madden D, Kwon T, McDowell C, Keating C, Balaraman V, de Carvahlo Madrid DM, Touchard L, Henningson J, Meade P, Krammer F, Morozov I, Richt JA, Driver JP*. Adjuvant Use of the Invariant-Natural-Killer-T-Cell Agonist α-Galactosylceramide Leads to Vaccine-Associated Enhanced Respiratory Disease in Influenza-Vaccinated Pigs. Vaccines (Basel). 2024 Sep 18;12(9):1068. doi: 10.3390/vaccines12091068. PMID: 39340098; PMCID: PMC11435877.

Monarch K, Yoon J, Uh K, Reese E, Restrepo DC, de Carvalho Madrid DM, Touchard L, Spate LD, Samuel MS, Driver JP, Lim JH, Schlink S, Whitworth KM, Wells KD, Prather RS, Chen PR, Lee K. Fetal bone engraftment reconstitutes the immune system in pigs with severe combined immunodeficiency. Lab Anim (NY). 2024 Oct;53(10):276-286. doi: 10.1038/s41684-024-01439-7. Epub 2024 Sep 17. PMID: 39289566; PMCID: PMC11439731.

Sparks Z, Wen Y, Hawkins I, Lednicky J, Abboud G, Nelson C, Driver JP*, Chauhan A. Sustained release of inactivated H1N1 virus from degradable microparticles for extended vaccination. Eur J Pharm Biopharm. 2024 Sep;202:114388. doi: 10.1016/j.ejpb.2024.114388. Epub 2024 Jun 28. PMID: 38945409.

Wen Y, Sparks Z, Hawkins I, Lednicky J, Abboud G, Nelson C, Chauhan A, Driver J*. Sustained release system from PLGA particles co-encapsulated with inactivated influenza virus with natural killer T cell agonist α-galactosylceramide. Eur J Pharm Biopharm. 2024 Aug;201:114365. doi: 10.1016/j.ejpb.2024.114365. Epub 2024 Jun 12. PMID: 38876362.

Casaro S, Prim JG, Gonzalez TD, Cunha F, Bisinotto RS, Chebel RC, Santos JEP, Nelson CD, Jeon SJ, Bicalho RC, Driver JP, Galvão KN. Integrating uterine microbiome and metabolome to advance the understanding of the uterine environment in dairy cows with metritis. Anim Microbiome. 2024 May 27;6(1):30. doi: 10.1186/s42523-024-00314-7. PMID: 38802977; PMCID: PMC11131188.

Ye C, Clements SA, Gu W, Geurts AM, Mathews CE, Serreze DV, Chen Y-G, Driver JP. Proc. Natl. Acad. Sci. USA. Deletion of Vb3⁺CD4⁺ T-cells by endogenous mouse mammary tumor virus 3 prevents type 1 diabetes induction by autoreactive CD8⁺ T-cells. Dec 5;120(49):e2312039120. doi: 10.1073/pnas.2312039120. Epub 2023 Nov 28.  PMID: 38015847; PMCID: PMC10710095

Casaro S, Prim JG, Gonzalez TD, Bisinotto RS, Chebel RC, Marrero MG, Silva ACM, Santos JEP, Nelson CD, Laporta J, Jeon SJ, Bicalho RC, Driver JP, Galvão KN. Unraveling the immune and metabolic changes associated with metritis in dairy cows. J Dairy Sci. 2023 Dec;106(12):9244-9259. doi: 10.3168/jds.2023-23289. Epub 2023 Aug 23. PMID: 37641354.

Ma W, Loving CL, Driver JP*. From Snoot to Tail: A Brief Review of Influenza Virus Infection and Immunity in Pigs. J Immunol. 2023 Oct 15;211(8):1187-1194. doi: 10.4049/jimmunol.2300385. PMID: 37782856; PMCID: PMC10824604.

Casaro S, Prim JG, Gonzalez TD, Figueiredo CC, Bisinotto RS, Chebel RC, Santos JEP, Nelson CD, Jeon SJ, Bicalho RC, Driver JP, Galvão KN. Blood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis. J Dairy Sci. 2023 Nov;106(11):8098-8109. doi: 10.3168/jds.2023-23433. Epub 2023 Aug 23. PMID: 37641346.

Oyebade AO, Lee S, Sultana H, Arriola K, Duvalsaint E, Nino De Guzman C, Fernandez Marenchino I, Marroquin Pacheco L, Amaro F, Ghedin Ghizzi L, Mu L, Guan H, Almeida KV, Rajo Andrade B, Zhao J, Tian P, Cheng C, Jiang Y, Driver J, Queiroz O, Ferraretto LF, Ogunade IM, Adesogan AT, Vyas D. Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows. J Dairy Sci. 2023 Dec;106(12):8611-8626. doi: 10.3168/jds.2022-22898. Epub 2023 Aug 23. PMID: 37641244.

Joyce S, Okoye GD, Driver JP. Die Kämpfe únd schláchten-the struggles and battles of innate-like effector T lymphocytes with microbes. Front Immunol. 2023 Apr 24;14:1117825. doi: 10.3389/fimmu.2023.1117825. PMID: 37168859; PMCID: PMC10165076.

Rocha CC, Silva FAC, Martins T, Marrero MG, Bromfield JJ, Driver J, Hansen T, Oliveira L, Binelli M. Culture of endometrial epithelial cells collected by a cytological brush in vivo. JDS Commun. 2022 Mar 16;3(3):217-221. doi: 10.3168/jdsc.2021-0189. PMID: 36338819; PMCID: PMC9623749.

Madrid DMC, Gu W, Artiaga BL, Yang G, Loeb J, Hawkins IK, Castleman WL, Lednicky JA, Richt JA, Driver JP*. Comparison of oseltamivir andα-galactosylceramide for reducing disease and transmission in pigs infected with 2009 H1N1 pandemic influenza virus. Front Vet Sci. 2022 Oct 20;9:999507. doi: 10.3389/fvets.2022.999507. PMID: 36337191; PMCID: PMC9635317.

Gu W, Madrid DMC, Joyce S, Driver JP. A single-cell analysis of thymopoiesis and thymic iNKT cell development in pigs. Cell Rep. 2022 Jul 5;40(1):111050. doi: 10.1016/j.celrep.2022.111050. PMID: 35793622; PMCID: PMC9704770.

Artiaga BL, Morozov I, Ransburgh R, Kwon T, Balaraman V, Indran SV, De Carvalho Madrid DM, Gu W, Henningson J, Ma W, Richt JA, Driver JP. Evaluating α-galactosylceramide as an adjuvant for live attenuated influenza vaccines in pigs. Anim Dis. 2022;2(1):19. doi: 10.1186/s44149-022-00051-x. Epub 2022 Aug 1. PMID: 35936354; PMCID: PMC9339466.

Casaro S, Marrero MG, Madrid DMC, Prim JG, Nelson CD, Galvão KN, Laporta J, Driver JP*. Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes. Vet Immunol Immunopathol. 2022 Jun;248:110417. doi:10.1016/j.vetimm.2022.110417. Epub 2022 Apr 2. PMID: 35413495.

Spencer TE, Wells KD, Lee K, Telugu BP, Hansen PJ, Bartol FF, Blomberg L, Schook LB, Dawson H, Lunney JK, Driver JP, Davis TA, Donovan SM, Dilger RN, Saif LJ, Moeser A, McGill JL, Smith G, Ireland JJ. Future of biomedical, agricultural, and biological systems research using domesticated animals. Biol Reprod. 2022 Apr 26;106(4):629-638. doi: 10.1093/biolre/ioac019. PMID: 35094055; PMCID: PMC9189970.

Marrero MG, Dado-Senn B, Field SL, Yang G, Driver JP, Laporta J. Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves. PLoS One. 2021 Jun 4;16(6):e0252474. doi: 10.1371/journal.pone.0252474. PMID: 34086766; PMCID: PMC8177632.

Ye C, Low BE, Wiles MV, Brusko TM, Serreze DV, Driver JP. CD70 Inversely Regulates Regulatory T Cells and Invariant NKT Cells and Modulates Type 1 Diabetes in NOD Mice. J Immunol. 2020 Oct 1;205(7):1763-1777. doi: 10.4049/jimmunol.2000148. Epub 2020 Aug 31. PMID: 32868408; PMCID: PMC7787366.

Marrero MG, Field SL, Skibiel AL, Dado-Senn B, Driver JP, Laporta J. Increasing serotonin bioavailability alters gene expression in peripheral leukocytes and lymphoid tissues of dairy calves. Sci Rep. 2020 Jun 16;10(1):9712. doi: 10.1038/s41598-020-66326-w. PMID: 32546841; PMCID: PMC7297988.

Zenobi MG, Gardinal R, Zuniga JE, Mamedova LK, Driver JP, Barton BA, Santos JEP, Staples CR, Nelson CD. Effect of prepartum energy intake and supplementation with ruminally protected choline on innate and adaptive immunity of multiparous Holstein cows. J Dairy Sci. 2020 Mar;103(3):2200-2216. doi: 10.3168/jds.2019-17378. Epub 2020 Jan 15. PMID: 31954584.

Driver JP, de Carvalho Madrid DM, Gu W, Artiaga BL, Richt JA. Modulation of Immune Responses to Influenza A Virus Vaccines by Natural Killer T Cells. Front Immunol. 2020 Oct 20;11:2172. doi: 10.3389/fimmu.2020.02172. PMID: 33193296; PMCID: PMC7606973.

Yang G, Artiaga BL, Lomelino CL, Jayaprakash AD, Sachidanandam R, Mckenna R, Driver JP. Next Generation Sequencing of the Pig αβ TCR Repertoire Identifies the Porcine Invariant NKT Cell Receptor. J Immunol. 2019 Apr 1;202(7):1981-1991. doi: 10.4049/jimmunol.1801171. Epub 2019 Feb 18. PMID: 30777925; PMCID: PMC6606045

Yang G, Driver JP, Van Kaer L. The Role of Autophagy in iNKT Cell Development. Front Immunol. 2018 Nov 14;9:2653. doi: 10.3389/fimmu.2018.02653. PMID: 30487800; PMCID: PMC6246678.

Driver JP, Racine JJ, Ye C, Lamont DJ, Newby BN, Leeth CM, Chapman HD, Brusko TM, Chen YG, Mathews CE, Serreze DV. Interferon-γ Limits Diabetogenic CD8+ T-Cell Effector Responses in Type 1 Diabetes. Diabetes. 2017 Mar;66(3):710-721. doi: 10.2337/db16-0846. Epub 2016 Dec 5. PMID: 27920091; PMCID: PMC5319715.

Driver JP, Lamont DJ, Gysemans C, Mathieu C, Serreze DV. Calcium insufficiency accelerates type 1 diabetes in vitamin D receptor-deficient nonobese diabetic (NOD) mice. Endocrinology. 2011 Dec;152(12):4620-9. doi: 10.1210/en.2011-1074. Epub 2011 Sep 27. PMID: 21952242; PMCID: PMC3230053

Driver JP, Chen YG, Zhang W, Asrat S, Serreze DV. Unmasking genes in a type 1 diabetes-resistant mouse strain that enhances pathogenic CD8 T-cell responses. Diabetes. 2011 Apr;60(4):1354-9. doi: 10.2337/db10-0885. Epub 2011 Feb 9. PMID: 21307079; PMCID: PMC3064110.

Driver JP, Scheuplein F, Chen YG, Grier AE, Wilson SB, Serreze DV. Invariant natural killer T-cell control of type 1 diabetes: a dendritic cell genetic decision of a silver bullet or Russian roulette. Diabetes. 2010 Feb;59(2):423-32. doi: 10.2337/db09-1116. Epub 2009 Nov 10. PMID: 19903740; PMCID: PMC2809954.

Madrid DMC, Gu W, Artiaga BL, Yang G, Loeb J, Hawkins IK, Castleman WL, Lednicky JA, Richt JA, Driver JP. Comparison of oseltamivir and α-galactosylceramide for reducing disease and transmission in pigs infected with 2009 H1N1 pandemic influenza virus. Front Vet Sci. 2022 Oct 20;9:999507. doi: 10.3389/fvets.2022.999507. PMID: 36337191; PMCID: PMC9635317.

Driver JP, Foreman O, Mathieu C, van Etten E, Serreze DV. Comparative therapeutic effects of orally administered 1,25-dihydroxyvitamin D(3) and 1alpha-hydroxyvitamin D(3) on type-1 diabetes in non-obese diabetic mice fed a normal-calcaemic diet. Clin Exp Immunol. 2008 Jan;151(1):76-85. doi: 10.1111/j.1365-2249.2007.03537.x. Epub 2007 Nov 5. PMID: 17983444; PMCID: PMC2276922.

Chen YG, Driver JP, Silveira PA, Serreze DV. Subcongenic analysis of genetic basis for impaired development of invariant NKT cells in NOD mice. Immunogenetics. 2007 Sep;59(9):705-12. doi: 10.1007/s00251-007-0236-4. Epub 2007 Jul 10. PMID: 17619875.

Driver JP, Atencio A, Pesti GM, Edwards HM Jr, Bakalli RI. The effect of maternal dietary vitamin D3 supplementation on performance and tibial dyschondroplasia of broiler chicks. Poult Sci. 2006 Jan;85(1):39-47. doi:10.1093/ps/85.1.39. PMID: 16493944.

Driver JP, Pesti GM, Bakalli RI, Edwards HM Jr. Effects of calcium and nonphytate phosphorus concentrations on phytase efficacy in broiler chicks. Poult Sci. 2005 Sep;84(9):1406-17. doi: 10.1093/ps/84.9.1406. PMID: 16206562.

Driver JP, Pesti GM, Bakalli RI, Edwards HM Jr. Phytase and 1alpha-hydroxycholecalciferol supplementation of broiler chickens during the starting and growing/finishing phases. Poult Sci. 2005 Oct;84(10):1616-28. doi: 10.1093/ps/84.10.1616. PMID: 16335132.